Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcobalamin II, Macrocytic Anemia (TCN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcobalamin II, Macrocytic Anemia (TCN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcobalamin II, Macrocytic Anemia (TCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcobalamin II, Macrocytic Anemia (TCN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve